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Sampling variation caused by A/D cards due to external trigger

F S Jaw1, C W Lin, C Y Wang

  • 1Center for Biomedical Engineering, College of Medicine, National Taiwan University, Taipei.

Journal of Neuroscience Methods
|November 1, 1995
PubMed
Summary
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Variations in evoked responses during electrophysiological recording were observed. A simulated model identified variable analog-to-digital (A/D) card sampling delays as the cause, suggesting hardware upgrades for accurate signal acquisition.

Area of Science:

  • Electrophysiology
  • Neuroscience
  • Biomedical Engineering

Background:

  • Analog-to-digital (A/D) converter cards are crucial for electrophysiological signal acquisition and analysis.
  • Observed variations in evoked responses across recording cycles complicate data interpretation.
  • Averaging multiple cycles can lead to reduced peak amplitude and increased duration of the resultant waveform.

Purpose of the Study:

  • To investigate the cause of signal variations in electrophysiological recordings.
  • To develop a simulated model to explain the observed phenomenon.
  • To identify potential solutions for improving signal acquisition accuracy.

Main Methods:

  • Development of a simulated sampling model for compound action potential.
  • Comparison of simulated model predictions with experimental electrophysiological data.

Related Experiment Videos

  • Analysis of the timing characteristics of the analog-to-digital (A/D) card's sampling process.

    • Main Results:

    • Experimental data showed strong agreement with the predictions of the simulated sampling model.
    • The primary cause of signal variation was identified as a long and variable delay between the external trigger and the initial sampling by the A/D card.
    • Post-acquisition programming could not resolve the identified timing issue.

    Conclusions:

    • Variable sampling delays in A/D cards are a significant source of error in electrophysiological recordings.
    • The simulated model effectively explains the observed waveform distortions.
    • Upgrading to a new A/D card is the most direct solution for laboratories experiencing these issues.