Ethanol stimulates diazepam binding inhibitor (DBI) mRNA expression in primary cultured neurons

Changes in expression of diazepam binding inhibitor (DBI) mRNA in cerebral cortical neurons following long-term ethanol (EtOH) exposure were examined. A significant increase in DBI mRNA expression was observed by the exposure of neurons to 50 mM EtOH for up to 5 days and to EtOH (1-100 mM) for 3 days. These EtOH-induced increases in DBI mRNA expression were further elevated after the additional cultivation of neurons under EtOH-free condition. beta-Actin mRNA expression was not altered by similar EtOH treatments. These results indicate that EtOH possesses the activity to increase the expression of DBI mRNA in cerebral cortical neurons.