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Related Experiment Videos

CD44 expression on epidermal melanocytes

K W Herbold1, J Zhou, J G Haggerty

  • 1Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

The Journal of Investigative Dermatology
|June 1, 1996
PubMed
Summary
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CD44 (chondroitin sulfate proteoglycan) is expressed on melanocytes, with two forms identified. This CD44 hematopoietic (CD44H) core protein functions as a part-time chondroitin sulfate proteoglycan.

Area of Science:

  • Cell Biology
  • Dermatology
  • Biochemistry

Background:

  • CD44 plays a role in cell adhesion and migration.
  • Melanoma cells exhibit altered CD44 expression compared to normal melanocytes.

Purpose of the Study:

  • To investigate CD44 expression on normal melanocytes.
  • To establish a baseline for CD44 expression in melanoma research.

Main Methods:

  • Immunofluorescence staining of melanocytes.
  • Analysis of CD44 protein forms using Western blotting.
  • Polymerase chain reaction (PCR) to detect CD44 splicing variants.
  • Treatment with 12-O-Tetradecanoylphorbol 13-acetate (TPA) to assess CD44 modification.

Main Results:

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  • CD44 is expressed across the melanocyte surface, concentrated at dendritic tips.
  • Two predominant CD44 forms exist: with and without chondroitin sulfate.
  • Only the CD44 hematopoietic (CD44H) core protein cDNA was detected.
  • TPA increased chondroitin sulfate chain size but not the proportion of modified CD44.
  • CD44 chondroitin sulfate proteoglycan constitutes 10% of total melanocyte proteoglycans.

Conclusions:

  • CD44H is expressed as a part-time chondroitin sulfate proteoglycan on normal cultured melanocytes.
  • Understanding normal CD44 expression provides a basis for melanoma studies.