[Optimization of a method of production of allergen extracts from ambrosia pollen]

Peculiarities of allergens extraction from the intact and destructed pollen grains of Ambrosia artemisiifolia L. have been studied, and the effect of the content of extracting buffer on stability of the obtained allergen extracts has been investigated. It is established that mechanical destruction of pollen during extraction permits decreasing protein output, increasing specific activity of the extracts and accelerating the procedure of their production. Introduction of some protective additions to the composition of the extracting buffer permits essentially increasing stability of the extracts under long-term storage at 4 degrees C.