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Posttranslational regulation of Ca(2+)-activated K+ currents by a target-derived factor in developing parasympathetic

P Subramony1, S Raucher, L Dryer

  • 1Programs in Neuroscience and Molecular Biophysics, Florida State University, Tallahassee 32306, USA.

Neuron
|July 1, 1996
PubMed

Insights

Chick ciliary ganglion (CG) neurons require target tissue interactions for normal expression of calcium-activated potassium (IK[Ca]) channels. Iris extracts promote IK[Ca channel expression in developing CG neurons.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Macroscopic calcium-activated potassium (IK[Ca]) channels are crucial for neuronal function.
  • IK[Ca] channel expression in chick ciliary ganglion (CG) neurons is regulated during development.
  • Synapse formation with target tissues plays a role in neuronal development.

Purpose of the Study:

  • To investigate the regulation of IK[Ca] channel expression in developing chick CG neurons.
  • To identify the molecular components involved in IK[Ca] channel expression.
  • To determine the role of target tissue interactions in IK[Ca] channel development.

Main Methods:

  • Isolation, cloning, and sequencing of chick CG slo partial cDNAs encoding IK[Ca] channels.
  • Detection of slo transcripts in developing CG neurons.
  • In vitro development of CG neurons with and without iris tissue extracts.
  • Electrophysiological recordings to measure whole-cell IK[Ca] currents.

Main Results:

  • Two chick CG slo partial cDNAs encoding IK[Ca] channels were identified.
  • slo transcripts were detected in CG neurons before target tissue interaction.
  • In vitro exposure to iris extracts induced normal whole-cell IK[Ca] expression.
  • Protein synthesis was not required for IK[Ca] expression; active component MW 40-60 kDa.

Conclusions:

  • Target tissue interactions, specifically with iris extracts, are essential for normal IK[Ca] channel expression in developing chick CG neurons.
  • The regulation of IK[Ca] channel expression is post-transcriptional and does not require de novo protein synthesis.
  • These findings provide insights into the molecular mechanisms underlying synapse formation and neuronal development.

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