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Bacillus subtilis spore coat assembly requires cotH gene expression

G Naclerio1, L Baccigalupi, R Zilhao

  • 1Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche, Avellino, Italy.

Journal of Bacteriology
|August 1, 1996
PubMed
Summary
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The Bacillus subtilis cotH gene is crucial for spore coat assembly. Its absence impairs spore germination and outer coat protein formation, highlighting its role in spore structure.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacillus subtilis endospores possess a protective protein coat.
  • This spore coat comprises an inner lamellar layer and an outer electron-dense layer.

Purpose of the Study:

  • To identify and characterize the function of the cotH gene in Bacillus subtilis spore coat formation.
  • To investigate the role of CotH in spore germination and coat protein assembly.

Main Methods:

  • Gene identification and characterization of cotH in Bacillus subtilis.
  • Construction and analysis of a cotH null mutant.
  • Assessment of spore resistance, germination, and coat protein composition in mutant strains.
  • Analysis of a cotE cotH double mutant.

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Main Results:

  • The cotH gene encodes a 42.8 kDa polypeptide.
  • cotH null mutant spores showed normal resistance but impaired germination.
  • Mutant spores were deficient in multiple coat proteins, including products of cotB, cotC, and cotG.
  • CotH appears to be involved in the assembly of outer coat proteins.

Conclusions:

  • The cotH gene plays a significant role in Bacillus subtilis spore coat assembly.
  • CotH is likely localized in the inner coat and influences the formation of the outer coat.
  • Proper assembly of the spore coat mediated by CotH is essential for normal spore germination.