Related Experiment Videos
Interleukin-8 gene expression in Staphylococcus aureus-infected endothelial cells
1Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 104611, USA.
Infection and Immunity
|August 1, 1996
Summary
Interleukin-8 (IL-8) is induced in endothelial cells infected with Staphylococcus aureus. This process promotes neutrophil migration to infection sites.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- Interleukin-8 (IL-8) is a key chemokine.
- IL-8 mediates neutrophil recruitment to sites of infection.
- Endothelial cells play a role in immune responses.
Purpose of the Study:
- To investigate the induction of IL-8 in endothelial cells.
- To understand the role of IL-8 in Staphylococcus aureus infection.
Main Methods:
- Endothelial cells were infected with Staphylococcus aureus.
- IL-8 mRNA and protein expression levels were measured.
Main Results:
- Staphylococcus aureus infection induced IL-8 mRNA expression in endothelial cells.
- Staphylococcus aureus infection induced IL-8 protein expression in endothelial cells.
- The expressed IL-8 promoted neutrophil transmigration across the endothelial cell monolayer.
Conclusions:
- Endothelial cells express IL-8 in response to Staphylococcus aureus infection.
- IL-8 produced by endothelial cells contributes to neutrophil recruitment during S. aureus infections.