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Related Experiment Videos

Trace elements in a commercial freeze-dried human urine reference material

C Veillon1, K Y Patterson

  • 1United States Department of Agriculture, Beltsville Human Nutrition Research Center, MD 20705, USA.

The Analyst
|July 1, 1996
PubMed
Summary

This study evaluated trace element levels in human urine reference material. Chromium showed potential contamination and inhomogeneity, while selenium was highly reproducible, indicating good homogeneity.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Biochemistry

Background:

  • Accurate trace element analysis is crucial for clinical diagnostics and environmental monitoring.
  • Standardized reference materials are essential for quality control in laboratories worldwide.
  • Seronorm Trace Elements Urine is a commercially prepared reference material for quality control.

Purpose of the Study:

  • To evaluate the trace element content of Seronorm Trace Elements Urine, Lot 101021.
  • To assess the homogeneity and potential contamination of the reference material.
  • To compare results obtained from different analytical methods.

Main Methods:

  • Isotope Dilution Mass Spectrometry (IDMS) and Atomic Absorption Spectrometry (AAS) were used for chromium and zinc determination.

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  • IDMS with enriched stable isotopes was used for selenium analysis.
  • Multielement analysis of vial and stopper leachates was performed to identify contaminant sources.

    • Main Results:

    • Chromium levels exhibited high relative standard deviation (21-25%), suggesting inhomogeneity or contamination, with half originating from the sample container.
    • Zinc concentrations increased over time after reconstitution, indicating potential leaching from the rubber stopper.
    • Selenium measurements by IDMS were highly reproducible (0.9% RSD), indicating good homogeneity and minimal contamination.

    Conclusions:

    • The human urine reference material shows variability for chromium and zinc, potentially due to container leaching and inhomogeneity.
    • Selenium appears to be a reliable analyte in this reference material due to its homogeneity and reproducibility.
    • Further investigation into container-related contamination is recommended for trace element reference materials.