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[The purification and analysis of S-antigen in rabbit retina]
Objective:
S-antigen from rabbit retina was isolated and purified to investigate the immune mechanisms of experimental autoimmune uveoretinitis (EAU) and lay a foundation for its experimental treatment.
Method:
Two-step ion exchange chromatography was used for the purification of S-antigen and it was analyzed.
Result:
The analytical studies show that the molecular weight of the purified S-antigen is 55,000, its isoelectric point is 6.40, and non-polar amino acid constitutes a relatively large proportion in its amino acids. Scanning tunnel electron microscope was used to investigate the configuration of S-antigen. It is elliptic globe-like. The purified S-antigen successfully induced the model of EAU.
Conclusion:
S-antigen purified by two-step ion exchange chromatography can be used for its own analytical studies and induction of EAU model.