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Updated: Aug 2, 2026

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
Published on: July 15, 2011
[Analysis of restriction fragment length polymorphism (RFLP) of Mycobacterium tuberculosis strains isolated from
N Lemaitre1, W Sougakoff, C Truffot
1Laboratoire de Bactériologie et Centre National de Référence de la Tuberculose et des infections à Mycobactéries atypiques, Groupe Hospitalier Pitié-Salpêtriére, Paris, France.
Abstract:
Analysis of restriction fragment length polymorphism (RFLP), using a DNA probe directed against the insertion sequence IS6110, was applied to strains of Mycobacterium tuberculosis successively isolated from four patients. In order to determine the cause of recurrence in these patients, the RFLP patterns of the corresponding isolated were analyzed. The profils obtained from the strains isolated from each of the patients were identical, thus suggesting that a relapse, rather than an exogenous reinfection with a new strain, was the cause of recurrence. The RFLP patterns of successive isolated remained unchanged during periods of time ranging from 5 months to 7 years, and were not modified after development of rifampin resistance. These results demonstrate the stability of the polymorphism detected by the IS6110 probe. Therefore, RFLP analysis is a powerfull epidemiologic tool to distinguish relapse from exogenous reinfection.
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