[Experimental study on "three-dimensional" culture of scar-derived fibroblasts]

Based on the monolayer cell culture system, we resuspended the scar-derived fibroblasts in a solution of polymerizing collagen to develop a "three-dimensional" cell culture system. In this system, cells, extracellular matrices and various modulating factors were associated to form an organic entity, in which the fibroblasts express their biologic characteristics similar to that in living body. With the three-dimensional system, we could study not only the morphology, proliferation and metabolism of fibroblasts, but also their cytophysiology, such as the role on connective tissue contraction. We recommend this culture system as a better model in the research of wound healing and scar formation.