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[A study on DNA content and cell cycle phase analysis in endometrial carcinoma]
Objective:
To study the prognostic value of DNA content, cell cycle phase in endometrial carcinoma.
Methods:
DNA content and cell cycle phase analysis were performed on 100 paraffin-embedded archival specimens from 103 cases of endometrial carcinoma using flow cytometry.
Results:
Aneuploidy was found in 71.0% of specimens. The aneuploidy rate in specimens from grade III, deep myometrial invasion and stage III, IV was significantly higher than that in specimens from grade I, nonmyometrial invasion and stage I (P < 0.05 respectively). 5-year survival rate for patients with aneuploidy was 65.9% and for patients with diploidy was 87.6% (P < 0.01). High S-phase fraction (SPF) was associated with high grade, deep myometrial invasion and advanced stage. The mean SPF was 13.4% in the living group and 17% in the dead group. The 5-year survival rate for patients with SPF > 17% was 50.4% and for patients with SPF < 17% was 79.1% (P < 0.01). SPF and stage were the most important prognostic factors in endometrial carcinoma by Cox's proportional hazards model.
Conclusions:
DNA ploidy and SPF were found to be of important prognostic indicators in endometrial carcinoma.