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[Autocrine transforming growth factor-beta stimulates human ovarian cancer cell growth in vitro and in vivo]
Objectives:
Our previous studies have shown that human ovarian cancer cell lines COC1 and COC2 secrete transforming growth factor-beta (TGF-beta) like substance in serum-free culture. In this study the effect of COC1 and COC2-produced TGF-beta like substance on COC1 and COC2 cell growth was investigated.
Methods:
The COC1 and COC2 RPMI 1640 serum-free conditioned media SFCM (SFCM1 from COC1, SFCM2 from COC2) were prepared, and their activities were investigated on COC1 and COC2 cell growth (SFCM1 on COC1, SFCM2 on COC2) in vitro and in vivo, and compared with that of TGF-beta.
Results:
SFCM1 and SFCM2 could separately promote COC1 and COC2 cell proliferation in culture with dose-dependent response, SFCM2 significantly promoted COC2 cell growth in BABL/C nude mice. Radioreceptor assay showed that both COC1 and COC2 expressed TGF-beta receptor (or binding site). The above mentioned SFCM growth-stimulating effects can be partially blocked by anti-TGF-beta antibody.
Conclusion:
The results suggest that there may be TGF-beta autocrine loop in the two ovarian cancer cell lines, which is associated with autonomous proliferation of COC1 and COC2 cells.