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Characterization and linkage mapping of ten sheep microsatellite markers derived from a sheep x hamster cell hybrid
J M Lumsden1, E A Lord, G W Montgomery
1Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Animal Genetics
|June 1, 1996
Abstract:
A cosmid library has been constructed from a sheep x hamster cell hybrid containing sheep chromosome t1, rob (6;24). Clones containing sheep DNA were identified by hybridizing to a total sheep genomic DNA probe. Small fragments (< 500 bp) containing (AC)n microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining microsatellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line.