Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Long-term low ascorbic acid intake reduces bone mass in guinea pigs

D E Kipp1, C E Grey, M E McElvain

  • 1Departments of Dietetics and Nutrition, University of Kansas Medical Center, Kansas City 66160, USA.

The Journal of Nutrition
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mild antiresorptive activity of an anti-vascular endothelial growth factor A antibody and sunitinib in a rat model of bone resorption.

Bone reports·2025
Same author

Intermittent parathyroid hormone enhances the healing of medication-related osteonecrosis of the jaw lesions in rice rats.

Frontiers in medicine·2023
Same author

Bone architecture, bone material properties, and bone turnover in non-osteoporotic post-menopausal women with fragility fracture.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2022
Same author

Preclinical models of medication-related osteonecrosis of the jaw (MRONJ).

Bone·2021
Same author

Biologic and pathologic aspects of osteocytes in the setting of medication-related osteonecrosis of the jaw (MRONJ).

Bone·2021
Same author

Preventing or controlling periodontitis reduces the occurrence of osteonecrosis of the jaw (ONJ) in rice rats (Oryzomys palustris).

Bone·2021

Long-term ascorbic acid (AA) deficiency in guinea pigs led to lower bone mass and increased bone turnover. Conversely, excess AA intake did not cause bone abnormalities, suggesting a protective effect against deficiency-related issues.

Area of Science:

  • Nutrition Science
  • Bone Biology
  • Skeletal Physiology

Background:

  • Ascorbic acid (AA), or vitamin C, is essential for collagen synthesis and bone health.
  • Understanding the long-term effects of varying AA intake on bone mass is crucial for skeletal development and maintenance.
  • Previous research has primarily focused on AA deficiency, with less known about the impact of long-term excess intake.

Purpose of the Study:

  • To investigate the long-term effects of low, normal, and excess dietary ascorbic acid (AA) intake on bone mass and turnover in guinea pigs.
  • To determine if prolonged AA deficiency or excess impacts skeletal maturation and bone mineral density.
  • To compare bone histomorphometry in guinea pigs with different long-term AA dietary levels.

Main Methods:

Related Experiment Videos

  • Young guinea pigs (12-14 days old) were fed diets with low (0.15 g/kg), normal (0.50 g/kg), or excess (10 g/kg) AA for 12 months.
  • Total body bone mineral density (BMD) and bone mineral content (BMC) were measured using dual-energy X-ray absorptiometry.
  • Histomorphometric analysis of tibial bone was performed after in vivo dual fluorochrome labeling to assess bone turnover rates.

    • Main Results:

    • Long-term low AA intake resulted in significantly lower total body BMD (4.9%) and BMC (12.4%) compared to the normal AA group.
    • Guinea pigs with low AA intake exhibited increased bone turnover, indicated by higher double-labeled bone surface, mineralizing surface, and bone formation rate.
    • No significant differences in BMD, BMC, or histomorphometric parameters were observed between the normal AA and excess AA groups, or between the low and excess AA groups.

    Conclusions:

    • Long-term ascorbic acid deficiency during critical growth and maturation phases leads to bone abnormalities and reduced bone mass in adult guinea pigs.
    • Sustained high dietary intake of ascorbic acid did not result in adverse bone effects and may not offer additional benefits beyond normal levels.
    • These findings highlight the importance of adequate ascorbic acid nutrition for maintaining skeletal health and underscore the lack of toxicity from excess intake within the studied range.