On-line detection of glutamate release from cultured chick retinospheroids

A continuous fluorometric assay was adapted to measure the release of endogenous glutamate from cultured chick retinospheroids. The results obtained with this technique are compared with the release of [3H]D-aspartate from monolayer cultures of chick retina cells. It is shown that although excitatory amino acids may be released in a Ca(2+)-dependent manner, most of the neurotransmitter release from cultured retina cells occurs by reversal of the glutamate transporter. The presence of extracellular Ca2+ may actually inhibit glutamate release by the cells present in the retinospheroids, or the [3H]D-aspartate release by cells in monolayers, when veratridine is the depolarizing agent.