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Updated: May 1, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Identification of retinolisomers by high-performance liquid chromatography not unequivocal up to now
Abstract:
Former publications dealing with high-performance liquid chromatography (HPLC) of retinol provide inconsistent results and lack evidence for the identification of isomers. For clarification of the problems in this field, the isomeric structure of the major retinol isomers in crystallized form was proven by 1H-NMR, UV absorption spectroscopy and by X-ray analysis. These identified isomeric forms of retinol were separated by HPLC with different eluent mixtures and the chromatograms compared in detail with previously published data. A conclusive assignment for HPLC separation of retinol isomers is given.
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