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A sequence-specific DNA-binding protein interacts with the xlnC upstream region of Streptomyces sp. strain EC3
F Giannotta1, J Georis, A Moreau
1Centre d'Ingénierie des Protéines, Université de Liége, Sart-Tilman, Belgium.
FEMS Microbiology Letters
|August 15, 1996
Abstract:
The alignment of the promoter region of several Streptomyces xylanases shows three conserved sequences which could be involved in gene regulation. By electromobility shift assays these specific sequences, present only in Streptomyces xylanolytic strains, were identified as protein-binding sites. The sequence required for efficient recognition by the retarding protein appeared to be a 4-bp inverted repeat: 5'-CTTT-Nx-AAAG-3'. The DNA-protein affinity was influenced by the culture conditions.