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High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium
M R Bogo1, M H Vainstein, F J Aragão
1Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
FEMS Microbiology Letters
|August 15, 1996
Abstract:
Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliae using benomyl resistance as a selectable marker. Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants micrograms-1 of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants micrograms-1 of DNA and 67% by electroporation; and 32 to 201 transformants micrograms-1 of DNA and 90% by biolistic. We demonstrate by PCR that 60% of the transformants were generated by gene conversion.