Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Horse complement protein C9: primary structure and cytotoxic activity

A F Esser1, R W Tarnuzzer, S Tomlinson

  • 1Department of Comparative and Experimental Pathology, University of Florida Health Science Center, Gainesville, USA.

Molecular Immunology
|May 1, 1996
PubMed
Summary

Horse C9 protein lacks hemolytic activity, but efficiently kills bacteria. Structural analysis reveals no obvious differences from hemolytic bovine C9, suggesting other factors influence red blood cell lysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type 1 Diabetes in care homes: A practical guide on management.

Diabetic medicine : a journal of the British Diabetic Association·2024
Same author

Perceptions of mental health providers of the barriers and facilitators of using and engaging youth in digital mental-health-enabled measurement based care.

Digital health·2024
Same author

A specialised pollination system using nectar-seeking thynnine wasps in Caladenia nobilis (Orchidaceae).

Plant biology (Stuttgart, Germany)·2019
Same author

Evaluation of multiplex tandem PCR (MT-PCR) assays for the detection of bacterial resistance genes among Enterobacteriaceae in clinical urines.

The Journal of antimicrobial chemotherapy·2018
Same author

Adherence to Adjuvant Endocrine Therapy in Christchurch Women with Early Breast Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2017
Same author

Regulation of the alternative pathway of complement modulates injury and immunity in a chronic model of dextran sulphate sodium-induced colitis.

Clinical and experimental immunology·2014

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Horse serum C9 (Complement 9) protein is known for its lack of hemolytic activity.
  • Understanding the molecular basis for this deficiency is crucial for comprehending complement system function.

Purpose of the Study:

  • To elucidate the molecular reasons behind the deficiency in hemolytic activity of horse C9.
  • To compare the structure and function of horse C9 with other species, particularly human and bovine C9.

Main Methods:

  • Cloning and sequencing of horse C9 cDNA from a horse liver cDNA library.
  • Purification of C9 protein from horse plasma and subsequent microsequencing.
  • Amino acid sequence analysis and comparison with human and bovine C9.

Related Experiment Videos

Main Results:

  • Horse C9 cDNA encodes a mature protein of 526 amino acids, 77% identical to human C9.
  • Identical domain structure and conserved regions, including the membrane interaction domain, were observed.
  • Horse C9 exhibits efficient cytotoxic activity against Gram-negative bacteria, despite lacking hemolytic activity.

Conclusions:

  • Horse C9 is a structurally sound molecule with potent antibacterial cytotoxic activity.
  • The inability of horse C9 to lyse erythrocytes may be due to interactions with regulatory proteins or target cell membranes, rather than intrinsic structural defects.