Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The TAL1/Scl basic helix-loop-helix protein blocks myogenic differentiation and E-box dependent transactivation

T J Hofmann1, M D Cole

  • 1Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.

Oncogene
|August 1, 1996
PubMed
Summary

The TAL1 oncogene, a genetic lesion in T-cell acute lymphoblastic leukemia, inhibits differentiation by binding to E2A proteins. This dominant inhibition mechanism is crucial for leukemia development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical profiling of the street cocktail drug 'nyaope' in South Africa using GC-MS II: Stability studies of the cannabinoid, opiate and antiretroviral components during sample storage.

Forensic science international·2019
Same author

Chemical profiling of the street cocktail drug 'nyaope' in South Africa using GC-MS I: Stability studies of components of 'nyaope' in organic solvents.

Forensic science international·2018
Same author

Occluded Solvent Analysis as a Basis for Heroin and Cocaine Sample Differentiation.

Forensic science review·2015
Same author

Forensic Analysis of Cathinones.

Forensic science review·2015
Same author

Antifungal polyoxygenated fatty acids fromAeollanthus parvifolius.

Journal of chemical ecology·2013
Same author

Burkitt's lymphoma-associated c-Myc mutations converge on a dramatically altered target gene response and implicate Nol5a/Nop56 in oncogenesis.

Oncogene·2013

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The TAL1 gene is frequently activated by chromosomal translocations in T-cell acute lymphoblastic leukemia (T-ALL).
  • TAL1 encodes a basic helix-loop-helix (bHLH) protein that binds DNA as a dimer with proteins like E2A.

Purpose of the Study:

  • To investigate the mechanism by which TAL1 inhibits differentiation and promotes T-ALL.
  • To determine the role of TAL1's C-terminal domain in its function.

Main Methods:

  • Ectopic expression of TAL1 in C3H10T1/2 cells.
  • Cotransfection assays with TAL1, myogenin, or E2-5 using reporter constructs.
  • Deletion analysis of the TAL1 C-terminal domain.

Main Results:

Related Experiment Videos

  • Ectopic TAL1 expression blocked myogenin-induced myotube differentiation.
  • TAL1 suppressed transcriptional activation by myogenin and E2-5.
  • Deletion of TAL1's C-terminal domain reduced or abolished transcriptional suppression.
  • TAL1's inhibitory function was comparable to that of Id protein.

Conclusions:

  • The C-terminal domain of TAL1 is critical for suppressing transcription and inhibiting differentiation.
  • TAL1 likely acts by masking the transactivation domain of E2A-related proteins.
  • Dominant inhibition of E2A proteins by TAL1 may be a key mechanism driving leukemia progression.