Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative electron spectroscopic imaging in bio-medicine: evaluation and application

A L Beckers1, E S Gelsema, W C De Bruijn

  • 1Department of Medical Informatics, Erasmus University Rotterdam, The Netherlands.

Journal of Microscopy
|July 1, 1996
PubMed
Summary

Electron spectroscopic imaging (ESI) allows chemical element mapping in biological tissues. This study validates ESI for quantifying iron in liver disease, showing its potential for detailed cellular analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of body position to quantify its effect on nocturnal blood pressure under ambulatory conditions.

Journal of hypertension·2001
Same author

Indicators of erythrocyte formation and degradation in rats with either vitamin A or iron deficiency.

The Journal of nutritional biochemistry·2000
Same author

Evaluation of techniques for the presentation of laboratory data: support of pattern recognition.

Methods of information in medicine·2000
Same author

Accumulation and release of iron in polarly and non-polarly cultured trophoblast cells isolated from human term placentas.

European journal of obstetrics, gynecology, and reproductive biology·1999
Same author

Characteristics of recovery from the euthyroid sick syndrome induced by tumor necrosis factor alpha in cancer patients.

Metabolism: clinical and experimental·1999
Same author

Structure, function and clinical significance of transferrin receptors.

Clinical chemistry and laboratory medicine·1999

Area of Science:

  • Materials Science
  • Biophysics
  • Analytical Chemistry

Background:

  • Energy-filtered transmission electron microscopy (EFTEM) with electron spectroscopic imaging (ESI) is crucial for analyzing chemical elements in biological samples.
  • Quantitative elemental mapping in ultrathin biological sections requires robust analysis techniques.
  • Iron storage diseases present challenges in visualizing and quantifying intracellular iron distribution.

Purpose of the Study:

  • To develop and validate an analysis technique for quantitative elemental mapping using ESI.
  • To evaluate the linearity, reproducibility, and visual interpretation of ESI measurements.
  • To assess the capability of ESI for detecting and characterizing iron in liver specimens from patients with iron storage diseases.

Main Methods:

Related Experiment Videos

  • Development of an analysis technique to calculate elemental maps and quantitative distributions from ESI sequences.
  • Evaluation of the measurement system using Fe-loaded Chelex beads to assess linearity and reproducibility.
  • Application of mass thickness correction for accurate quantification and visual interpretation of element distributions.
  • Testing iron detectability and characterization in liver specimens from a patient with iron storage disease.

Main Results:

  • The ESI concentration measurement scale demonstrates approximate linearity up to a relative section thickness of 0.5.
  • Reproducibility measurements showed a 6.4% difference in iron concentration between serial measurements using Fe-Chelex.
  • Mass thickness correction is essential for accurate quantification and visual interpretation, while signal-to-noise ratio is key for element detection.
  • ESI successfully demonstrated quantitative chemical analysis capabilities, distinguishing different iron components in liver cells of an iron-overloaded patient.

Conclusions:

  • The developed ESI analysis technique provides quantitative chemical information for biological samples.
  • ESI is a valuable tool for investigating iron storage diseases, enabling the characterization of iron-containing components at the cellular level.
  • Monitoring relative section thickness is important for maintaining the linearity of ESI concentration measurements.