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Related Experiment Videos

A role for CD9 molecules in T cell activation

X G Tai1, Y Yashiro, R Abe

  • 1Biomedical Research Center, Osaka University Medical School, Japan.

The Journal of Experimental Medicine
|August 1, 1996
PubMed
Summary

Researchers discovered CD9 as a novel T cell costimulatory molecule. This finding reveals CD9 provides a potent signal for T cell activation, independent of the known CD28 pathway, enhancing our understanding of immune responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD28 molecule-mediated costimulation is crucial for T cell activation.
  • CD28-deficient mice can still mount T cell-dependent immune responses, indicating alternative costimulatory pathways exist.

Purpose of the Study:

  • To identify novel costimulatory molecules on T cells beyond CD28.
  • To characterize the function and mechanism of a newly identified costimulatory molecule.

Main Methods:

  • Development of a monoclonal antibody (mAb) to identify costimulatory molecules.
  • Expression cloning to identify the target molecule of the mAb.
  • T cell proliferation assays using CD28-deficient and wild-type mice.

Main Results:

  • A novel molecule, identified as CD9, was found to costimulate T cells.

Related Experiment Videos

  • Anti-CD9 mAb induced T cell proliferation, synergizing with suboptimal anti-CD3 mAb doses.
  • CD9-mediated costimulation was potent and functioned independently of CD28.

    • Conclusions:

    • CD9 acts as a significant costimulatory molecule on T cells.
    • CD9 provides a potent, CD28-independent costimulatory signal for T cell activation.
    • This discovery opens new avenues for understanding and manipulating T cell responses.