Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conservation patterns in angiosperm rDNA ITS2 sequences

M A Hershkovitz1, E A Zimmer

  • 1Laboratory of Molecular Systematics, MRC534, Smithsonian Institution, Washington, DC 20560, USA.

Nucleic Acids Research
|August 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Baryum Wander, a new contrast substance with particular properties for the examination of the digestive tract].

Bulletin des actualites medicales·2014
Same author

Genomic organization of the ribosomal DNA of sorghum and its close relatives.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

50 Years of medical radiology.

Experientia·2010
Same author

The X-ray of the small intestine.

Gastroenterologia·2010
Same author

Unraveling the evolutionary radiation of the families of the Zingiberales using morphological and molecular evidence.

Systematic biology·2002
Same author

Evolution of insular Pacific Pittosporum (Pittosporaceae): origin of the Hawaiian radiation.

Molecular phylogenetics and evolution·2002
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Internal transcribed spacers (ITS1 and ITS2) in nuclear ribosomal DNA provide valuable data for plant phylogenetic analyses. The ITS2 region is alignable across angiosperm families, aiding in lineage diagnosis and revealing secondary structure insights.

Area of Science:

  • Molecular Biology
  • Phylogenetics
  • Plant Science

Background:

  • Nuclear ribosomal DNA, specifically internal transcribed spacers (ITS1 and ITS2), is widely used for phylogenetic studies in angiosperms and other eukaryotes.
  • The utility of ITS sequences lies in their informative variation at interspecific and intergeneric levels.

Purpose of the Study:

  • To assess the alignability and diagnostic potential of the ITS2 region for phylogenetic analyses in angiosperms.
  • To investigate the base composition and secondary structure of angiosperm ITS2 sequences.

Main Methods:

  • Sequence alignment of ITS2 regions from various angiosperm taxa.
  • Phenetic analysis to evaluate the diagnostic power of ITS2 data.
  • Base compositional analysis to characterize ITS2 sequence properties.

Related Experiment Videos

  • Secondary structure modeling of ITS2 to identify conserved pairing relationships.

    • Main Results:

    • One-third to one-half of the ITS2 sequence is alignable above the family level in angiosperms.
    • Phenetic analysis demonstrates that ITS2 contains sufficient information to diagnose lineages at multiple hierarchical levels.
    • Angiosperm ITS2 is inherently GC-rich, with a more variable proportion of T compared to other bases.
    • A general model of angiosperm ITS2 secondary structure reveals common pairing in conserved regions, with variations suggesting non-paired compensatory mutations.

    Conclusions:

    • The ITS2 region is a highly informative marker for angiosperm phylogenetics, even at higher taxonomic levels.
    • ITS2 secondary structure analysis provides insights into evolutionary processes, including non-paired compensatory mutations.