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Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis

F Ginetti¹, M Perego, A M Albertini

¹Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Italy.

Microbiology (Reading, England)

|August 1, 1996

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The Bacillus subtilis mutS and mutL genes are crucial for DNA mismatch repair. Deleting these genes increases mutation frequency and affects transformation.

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

The DNA mismatch repair system corrects errors during DNA replication.
The Bacillus subtilis mutS and mutL genes are key components of this system.

Purpose of the Study:

To clone and characterize the Bacillus subtilis mutS and mutL genes.
To investigate the role of these genes in DNA repair and mutation frequency.

Main Methods:

Gene cloning and characterization.
Sequence analysis of mutS and mutL.
Deletion mutagenesis to create mutS and mutL knockouts.
Construction of a mutSL-lacZ transcriptional fusion to study gene expression.

Main Results:

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The mutS and mutL genes are organized in a single operon downstream of the cotE gene.
The deduced MutS and MutL proteins show significant identity to their Streptococcus pneumoniae counterparts (HexA and HexB).
Deletion of mutS and mutL led to increased spontaneous mutation rates and abolished the marker effect in transformation.
mut operon expression increased during late exponential growth.

Conclusions:

The Bacillus subtilis mutS and mutL genes are essential for maintaining genomic stability.
Their operon structure and expression patterns suggest coordinated regulation.
These findings contribute to understanding DNA repair mechanisms in bacteria.