[Rapid detection of Salmonella with MUCAP reagent]

The 4-methylumbelliferyl-caprylate (MUCAP) was applied for the identification of Salmonella, it's specification, sensitity, and availability were reported. 65 strains of standard Salmonella, 48 strains of Salmonella isolated from foods growing on plates of HE, DHL, SS and MaConkey agar have been tested with MUCAP. All of them were identified as MUCAP positive; whereas, among the non-Salmonella tested only Pseudamonas spp., Aeromonas hydrophilia and Plesiomonas shigelloides were shown as positive, but they could be differentiated easily with Salmonella by means of oxidase test. The unspecific reaction of Serratia marcescens could be eliminated by using the plate medium which has been added of 1% sucrose. Both the sensitivity and specificity of the method were up to 97% more, and the operating of the method was very facility and to be done within few minutes.