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[Ultrathin sections in postmortem morphological diagnosis of kidney diseases]
Abstract:
Authors for the light-microscopic examination of various--mainly glomerular--diseases of kidneys in addition to paraffin section have used also ultrathin sections (0,5 mu). Material was fixed in buffered-formalin solution and embedded in metacrylate or Durcupan ACM. Sections obtained by this method can be studied by electron micro-scope as well. Sections were stained with methylene-blue and basicfuchsine, further with periodic-acid silver methenamine. The use of ultrathin sections for the light-microscopic studies can not replace the study of paraffin-sections. Ultrathin sections are suitable for the detection of fine details. They can be studied by light- and electron microscopy as well.
Insights
Ultrathin sections offer detailed insights for light microscopy of kidney diseases but do not replace traditional paraffin sections. These ultrathin sections are also compatible with electron microscopy, enhancing diagnostic capabilities.
Area of Science:
- Nephrology
- Histopathology
- Microscopy