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Updated: Jul 27, 2026
16:33
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
Published on: February 18, 2013
[Adipsin system--acylation-stimulation protein (ASP) and hyperapo-B]
Q Rato1, K Cianflone, A Sniderman
1Serviço de Cardiologia, Hospital de São Bernardo, Setúbal.
Summary
Acylation Stimulating Protein (ASP) regulates triglyceride clearance. Lower ASP function leads to hyperapoB and increased risk of coronary artery disease.
Area of Science:
- Biochemistry
- Human Physiology
- Lipid Metabolism
Background:
- Acylation Stimulating Protein (ASP) is a potent stimulant of triglyceride synthesis.
- The adipsina-ASP system plays a role in plasma triglyceride clearance.
- Dyslipoproteinemias are associated with premature coronary artery disease.
Purpose of the Study:
- To investigate the in vivo role of the adipsina-ASP system in triglyceride clearance.
- To explore the link between adipsina-ASP system function and hyperapoB.
- To understand the contribution of adipsina-ASP system defects to dyslipoproteinemias.
Main Methods:
- In vivo studies in humans.
- Measurement of fasting and postprandial plasma ASP levels.
- Assessment of triglyceride clearance rates.
- Analysis of lipoprotein particle secretion.
Main Results:
- Higher fasting and peak ASP plasma levels correlate with faster triglyceride clearance.
- Decreased adipsina-ASP system function increases free fatty acid and chylomicron remnant delivery to the liver.
- Defective adipsina-ASP system function contributes to hyperapoB secretion.
Conclusions:
- The adipsina-ASP system is crucial for efficient triglyceride clearance from plasma.
- Impaired adipsina-ASP system function is a cause of hyperapoB.
- Defects in this system contribute to dyslipoproteinemias and increase coronary artery disease risk.
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