Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Replication fork arrest at relocated replication terminators on the Bacillus subtilis chromosome

A H Franks¹, R G Wake

¹Department of Biochemistry, University of Sydney, New South Wales, Australia.

Journal of Bacteriology

|July 1, 1996

Summary

The Bacillus subtilis terC region

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The impact of single cysteine residue mutations on the replication terminator protein.

Biochemical and biophysical research communications·2003

Same author

Autosomal dominant hypocalcemia: a novel activating mutation (E604K) in the cysteine-rich domain of the calcium-sensing receptor.

The Journal of clinical endocrinology and metabolism·2003

Same author

L-amino acid sensing by the calcium-sensing receptor: a general mechanism for coupling protein and calcium metabolism?

European journal of clinical nutrition·2002

Same author

A rapid, semi-automated method for detection of Galbeta1-4GlcNAc alpha2,6-sialyltransferase (EC 2.4.99.1) activity using the lectin Sambucus nigra agglutinin.

Glycobiology·2001

Same author

Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest.

Nature structural biology·2001

Same author

Mid-cell Z ring assembly in the absence of entry into the elongation phase of the round of replication in bacteria: co-ordinating chromosome replication with cell division.

Molecular microbiology·2000

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

The replication terminus region of Bacillus subtilis, known as terC, contains TerI and TerII sites crucial for regulating DNA replication.
Previous studies relocated terC to the clockwise-replicating segment, but its function on both segments remained unclear.

Purpose of the Study:

To investigate the functionality of the terC region, specifically TerI and TerII, in replication fork arrest when relocated to both clockwise and anticlockwise chromosomal segments.
To determine the role of replication terminator protein (RTP) levels in TerII-mediated fork arrest.

Main Methods:

Relocation of the terC region to specific chromosomal locations (serC and cym) on both replicating segments.
Analysis of replication fork arrest using marker frequency analysis.
Investigating the effect of RTP overproduction on TerII functionality.

Main Results:

TerI functions in fork arrest only when oriented against the approaching replication fork.
TerII is nonfunctional in fork arrest in vivo due to limiting RTP levels, but can be activated by RTP overproduction.
TerI causes transient arrest, leading to replication forks meeting around 145 degrees and cell elongation.

Conclusions:

The orientation of terC elements is critical for their function in replication termination.
In vivo functionality of TerII is dependent on sufficient RTP levels.
TerI-mediated arrest significantly impacts replication timing and can delay cell division in Bacillus subtilis.

Related Experiment Videos