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cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos
1Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France.
Abstract:
We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter.