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Updated: Aug 11, 2026

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Published on: January 17, 2012
Grb10: A new substrate of the insulin-like growth factor I receptor
A Morrione1, B Valentinis, S Li
1Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Abstract:
Using the yeast two-hybrid system, we have isolated a new substrate of the insulin-like growth factor I receptor (IGF-IR), identified as Grb10, a member of the family of SH2 domain proteins. With the help of several mutants of the IGF-IR, we have mapped the binding site of Grb10 between amino acids 1229 and 1245 of the receptor, a sequence that is dispensable for the mitogenic activity of the IGF-IR. Grb10 coprecipitates with the IGF-IR in cell lysates and is probably involved in the regulation of its activity.
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