Transient outward and delayed rectifier currents in canine atrium: properties and role of isolation methods

Although the dog is the principal species used for in vivo studies of atrial arrhythmias, little is known about currents governing canine atrial repolarization. Cells were isolated from dog atria by exposure to collagenase of tissue in vitro ("chunk cells") and by arterial perfusion ("perfusion cells"). Whole cell voltage clamp revealed transient outward K+ current (Ito1), Ca(2+)-dependent Cl- current (Ito2), and delayed rectifier K+ current (IK). Ito1 recovered rapidly and showed little frequency dependence. Two components of IK were present as follows: a rapidly activating E-4031-sensitive current with marked inward recitification and a slower-activating E-4031-insensitive component. Ito1 and IK resembled corresponding currents previously described in human atrium. Transient outward currents were similar in chunk and perfusion cells, but IK was seen in 4% of chunk cells vs. 99% of perfusion cells (P < 0.001). Suppression of each identified current retarded canine action potential repolarization. We conclude that Ito1, Ito2, and both components of IK are present in dog atrium, IK is much more sensitive to the isolation method than Ito1 or Ito2, and the properties of two important repolarizing currents (Ito1 and IK) previously described in human atrium are similar to those in dog atrium.