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A minimal medium for growth of Pasteurella multocida
P E Jablonski1, M Jaworski, C J Hovde
1Department of Biological Sciences, Northern Illinois University, DeKalb 60115-2861, USA. Pjablonski@niu.edu
Abstract:
We developed a minimal medium supporting the growth of both toxigenic and nontoxigenic strains of Pasteurella multocida to optical densities of > 0.5 (600 nm). P. multocida P1059 (ATCC 15742), one of a number of strains which can cause fowl cholera, was used as the model strain in this study. The medium was composed of 17 ingredients including cysteine, glutamic acid, leucine, methionine, inorganic salts, nicotinamide, pantothenate, thiamine, and an energy source. Leucine was not required for growth but was stimulatory, and thiamine could be replaced by adenine. An additional 46 strains of P. multocida were tested, and 40 out of 46 (87%) strains grew as well as strain P1059 through a minimum of 10 serial transfers. P. multocida toxin (PMT) was produced when cells of a known toxigenic strain (P4261) were cultivated in the minimal medium. No growth of Pasteurella Haemolytica or Pasteurella trehalosi strains was observed in this minimal medium.
Insights
Researchers developed a novel minimal medium supporting Pasteurella multocida growth. This medium enables Pasteurella multocida toxin production and supports 87% of tested strains, aiding fowl cholera research.
Area of Science:
- Microbiology
- Bacteriology
- Animal Health
Background:
- Pasteurella multocida is an important bacterial pathogen causing significant diseases in animals, including fowl cholera.
- The lack of a defined minimal medium has hindered detailed studies on P. multocida physiology and virulence factor production.
Purpose of the Study:
- To develop and characterize a minimal medium that supports the growth of both toxigenic and nontoxigenic Pasteurella multocida strains.
- To assess the utility of the medium for Pasteurella multocida toxin (PMT) production and its broad applicability across different strains.
Main Methods:
- Formulation of a 17-ingredient minimal medium using P. multocida P1059 (ATCC 15742) as a model.
- Testing the growth of 46 additional P. multocida strains and evaluating growth through serial transfers.
- Cultivating a toxigenic P. multocida strain in the medium to assess Pasteurella multocida toxin production.
Main Results:
- The developed minimal medium supported growth of P. multocida to optical densities > 0.5 (600 nm).
- 87% (40/46) of tested P. multocida strains demonstrated robust growth comparable to the model strain over 10 serial transfers.
- Pasteurella multocida toxin (PMT) was successfully produced by a toxigenic strain in the minimal medium.
- No growth was observed for Pasteurella haemolytica or Pasteurella trehalosi strains in this medium.
Conclusions:
- A defined minimal medium has been established for Pasteurella multocida, facilitating research into its growth requirements and virulence.
- The medium supports a high percentage of P. multocida strains and enables toxin production, making it a valuable tool for studying fowl cholera and related infections.