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Apoptosis is associated with increased cell surface tissue factor procoagulant activity

E W Greeno1, R R Bach, C F Moldow

  • 1Department of Medicine, University of Minnesota, Minneapolis, USA.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|August 1, 1996
PubMed
Summary

Apoptosis, a programmed cell death process, enhances the activity of tissue factor (TF), a key initiator of blood clotting. This finding links cell death pathways to coagulation, impacting wound healing and cancer.

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Area of Science:

  • Cell Biology
  • Hematology
  • Biochemistry

Background:

  • Tissue factor (TF) initiates coagulation but is typically inactive on intact cell surfaces (TF encryption).
  • Apoptosis involves cell membrane changes and has been linked to TF de-encryption and increased procoagulant activity.

Purpose of the Study:

  • To investigate the hypothesis that apoptosis leads to enhanced TF procoagulant activity.
  • To explore the relationship between apoptosis and the expression of cell surface TF activity.

Main Methods:

  • Cultured human fibroblasts and endothelial cells were induced into apoptosis using specific treatments.
  • Morphologic and DNA changes were assessed to confirm apoptosis.
  • Changes in cell surface TF activity levels were measured under apoptotic conditions.

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Main Results:

  • Apoptosis induction in endothelial cells correlated with TF de-encryption and increased activity.
  • Fibroblasts undergoing apoptosis also showed increased TF activity, primarily linked to morphologic changes.
  • A consistent association was observed between apoptosis and the expression of cell surface TF activity.

Conclusions:

  • Apoptosis is associated with the de-encryption and enhanced procoagulant activity of cell surface tissue factor.
  • This link between apoptosis and TF activity may play a role in regulating cell turnover during tissue remodeling.
  • Potential implications for understanding coagulation in wound healing and neoplasia.