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Cloning of mouse prolidase cDNA: predominant expression of prolidase mRNA in kidney
T Ishii1, S Tsujino, S Matsunobu
1Department of Inherited Metabolic Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Abstract:
We cloned mouse prolidase cDNA from a mouse liver cDNA library. Homology to human prolidase is 83.2% at the nucleotide level and 87.2% at the amino acid level. Northern blot analysis showed that while prolidase mRNA was transcribed in brain, heart, liver, and muscle, it was predominantly transcribed in kidney.
Insights
Researchers cloned mouse prolidase cDNA, finding high homology to human prolidase. Prolidase mRNA is primarily transcribed in the kidney, suggesting a key role for this organ.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Prolidase (EC 3.4.11.2) is a metalloenzyme crucial for collagen metabolism and imidodipeptide hydrolysis.
- Understanding prolidase gene expression is vital for its role in biological processes.
Purpose of the Study:
- To clone mouse prolidase cDNA and investigate its expression patterns.
- To determine the homology between mouse and human prolidase.
Main Methods:
- Cloning of mouse prolidase cDNA from a mouse liver cDNA library.
- Northern blot analysis to detect prolidase mRNA expression in various mouse tissues.
Main Results:
- Mouse prolidase cDNA was successfully cloned.
- High homology observed between mouse and human prolidase at nucleotide (83.2%) and amino acid (87.2%) levels.
- Prolidase mRNA expression was detected in brain, heart, liver, and muscle, with predominant transcription in the kidney.
Conclusions:
- The cloned mouse prolidase exhibits significant homology to its human counterpart.
- Kidney is identified as the primary site of prolidase gene expression in mice, indicating a potentially significant role in renal function.