Related Experiment Videos
Change of complement C1s synthesis during development of hamster cartilage
T Toyoguchi1, K Yamaguchi, K Nakagawa
1Division of Oncology and Biology, National Institute of Radiological Sciences 4-9-1, Anagawa, Inage-ku, Chiba 263, Japan.
Abstract:
Expression of the first complement component (C1s) has been examined in chondrocytes of hamster epiphyseal cartilage during development and fracture healing. C1s is immunostained with anti-hamster C1s monoclonal antibody, PG11. The C1s staining increases in accordance with chondrocyte differentiation and reaches a maximal level in hypertrophic chondrocytes. This change is observed at both the tibia ossification center and at the callus in which the replacement of cartilage by bone marrow takes place. The concomitant increase of C1s and chondrocyte hypertrophy has been confirmed by RNA blot and by in situ hybridization. These results, in addition to previous findings on C1s collagenolytic and gelatinolytic activities, suggest C1s participation in cartilage remodeling.