[Determination of 2,4-dichlorophenoxyacetic acid using monoclonal antibodies and a biotin-streptavidin detection...

Simple methods to determine 2,4-dichlorophenoxyacetic acid (2,4-D) using biotinylated monoclonal antibodies in combination with a highly active streptavidin-peroxidase conjugate were developed on the basis of the immunofiltration dot assay and competitive enzyme immunoassay (EIA). The detection limit of 2,4-D acid for immunofiltration and EIA was 2-3 ng/ml, the total assay duration being 5 min and 1.5 h, respectively.