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Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger
K Kirimura1, T Matsui, S Sugano
1Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan.
FEMS Microbiology Letters
|August 1, 1996
Abstract:
The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.