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Related Experiment Videos

Starvation-induced decrease in the maximal binding capacity for triiodothyronine of the thyroid hormone receptor is

T Tagami1, H Nakamura, S Sasaki

  • 1Department of Internal Medicine, Kyoto University School of Medicine, Japan.

Metabolism: Clinical and Experimental
|August 1, 1996
PubMed
Summary
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Starvation reduces thyroid hormone receptor (TR) binding capacity in rat liver. This decrease is linked to lower TR protein levels, not mRNA, suggesting protein reduction drives altered T3 binding during fasting.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cellular Biology

Background:

  • Thyroid hormone receptor (TR) mediates biological responses to thyroid hormones.
  • Alterations in TR's maximal triiodothyronine (T3)-binding capacity (Cmax) occur in pathophysiological states.
  • TR mRNA levels do not consistently correlate with Cmax, as seen in fasting models.

Purpose of the Study:

  • To investigate starvation-induced changes in thyroid hormone receptor (TR) protein levels and T3-binding capacity.
  • To determine if changes in TR protein or mRNA are responsible for reduced T3 binding during starvation.

Main Methods:

  • Western blot analysis using anti-TR(alpha 1 + beta)antiserum to detect TR proteins.
  • Scatchard plot analysis to measure T3-binding capacity (Cmax).

Related Experiment Videos

  • Study conducted on rat liver following 72 hours of starvation.

    • Main Results:

    • Starvation for 72 hours decreased hepatic TR Cmax to 72.5% of control levels.
    • Western blot revealed decreased levels of 47-kd and 55-kd TR proteins to 64% and 66% of control values, respectively.
    • Changes in Cmax and TR protein levels correlated with reduced total hepatic nuclear protein concentration.

    Conclusions:

    • The decrease in T3-binding activity of the TR during starvation is attributed to a reduction in TR protein levels.
    • These findings highlight the role of TR protein quantity in regulating thyroid hormone action under metabolic stress.