Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ICRF-193 modifies etoposide-induced apoptosis in thymocytes

C Tanimoto1, S Hirakawa, H Kawasaki

  • 1Third Department of Internal Medicine, Okayama University Medical School, Japan.

Acta Medica Okayama
|December 1, 1995
PubMed
Summary

Etoposide (VP-16), a topoisomerase II inhibitor, induces apoptosis in mouse thymocytes. Another inhibitor, ICRF-193, partially blocked VP-16

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanism of haematuria in lupus nephritis.

Annals of the rheumatic diseases·1995
Same author

[Prevalence and transmission of hepatitis C virus in polluted area in Japan].

Nihon rinsho. Japanese journal of clinical medicine·1995
Same author

[Changes of serum 2',5'-oligoadenylate synthetase activity and neopterin during interferon treatment for chronic hepatitis C].

Nihon rinsho. Japanese journal of clinical medicine·1995
Same author

Use of haloacetate dehalogenase genes as selection markers for Escherichia coli and Pseudomonas vectors.

Biodegradation·1995
Same author

Diagnosis of vascular compression in facial spasm by stereoscopic short-range magnetic resonance angiography. Technical note.

Journal of neurosurgery·1995
Same author

The effect of a TXA2 receptor antagonist ON-579 on experimental allergic reactions.

Prostaglandins, leukotrienes, and essential fatty acids·1995

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Pharmacology

Background:

  • Etoposide (VP-16) is a topoisomerase II (TopoII) inhibitor that induces apoptosis.
  • VP-16 stabilizes a cleavable enzyme-DNA complex, leading to DNA damage.
  • The precise mechanism of VP-16-induced apoptosis requires further elucidation.

Purpose of the Study:

  • To investigate the role of DNA-strand breaks in VP-16-induced apoptosis.
  • To clarify the mechanism of action of VP-16 using a non-cleavable complex-forming TopoII inhibitor, ICRF-193.
  • To compare the apoptotic effects of VP-16 and ICRF-193 in murine thymocytes.

Main Methods:

  • In vitro treatment of murine thymocytes with VP-16 and/or ICRF-193.
  • Assessment of DNA fragmentation as a marker of apoptosis.

Related Experiment Videos

  • Analysis of the time course of DNA fragmentation induced by each inhibitor.

    • Main Results:

    • ICRF-193 partially inhibited VP-16-induced DNA fragmentation in early stages.
    • The inhibitory effect of ICRF-193 diminished over time.
    • ICRF-193 alone induced apoptosis in murine thymocytes, with a distinct time course compared to VP-16.

    Conclusions:

    • TopoII inhibitors can induce apoptosis through pathways involving DNA-strand breaks (early stage) and potentially independent of them.
    • VP-16 and ICRF-193 exhibit distinct mechanisms and kinetics in inducing thymocyte apoptosis.
    • These findings provide insights into the complex mechanisms of TopoII inhibitor-mediated cell death.