Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interleukin gene expression in mouse preimplantation development

N Gerwin1, G Q Jia, R Kulbacki

  • 1Center for Blood Research, Inc., Boston, MA 02115, USA.

Developmental Immunology
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

US immigration order strikes against biotech.

Nature biotechnology·2017
Same author

Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat.

Poultry science·2012
Same author

Histopathology atlas of animal model systems - overview of guiding principles.

Osteoarthritis and cartilage·2010
Same author

The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat.

Osteoarthritis and cartilage·2010
Same author

Growth plate cartilage as developmental model in osteoarthritis research--potentials and limitations.

Current drug targets·2007
Same author

Uniformly carbon-covered alumina and its surface characteristics.

Langmuir : the ACS journal of surfaces and colloids·2005

Cytokines like Interleukin-1 (IL-1) and Tumor Necrosis Factor alpha (TNF alpha) are expressed in early mouse embryos. These growth factors and their receptors play a role in mammalian preimplantation development.

Area of Science:

  • Developmental Biology
  • Immunology
  • Reproductive Biology

Background:

  • Mammalian embryogenesis relies on growth factors for development.
  • Cytokines are key polypeptide growth factors involved in cellular processes.

Purpose of the Study:

  • Identify novel cytokine family members involved in preimplantation embryo growth and differentiation.
  • Investigate the expression of cytokines and their receptors during early mouse development.

Main Methods:

  • Studied gene expression patterns of cytokines and receptors in vitro during mouse preimplantation development.
  • Utilized poly(A)+ mRNA analysis and immunostaining with monoclonal antibodies.
  • Examined expression in unfertilized oocytes and various preimplantation stages.

Related Experiment Videos

Main Results:

  • Found differential expression of mRNAs for IL-1, IL-3, IL-6, IL-7, and TNF alpha.
  • Confirmed translation of IL-1, IL-6, and TNF alpha mRNAs into proteins.
  • Detected positive staining for IL-1 and IL-6 receptors in preimplantation embryos.

Conclusions:

  • "Inflammatory-type" cytokines and their receptors are expressed during early mouse development.
  • Suggests a significant role for these cytokines in mammalian preimplantation ontogeny.
  • Highlights the importance of cytokine signaling in early embryonic growth and differentiation.