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Updated: Aug 15, 2026

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
Published on: December 30, 2015
Germline transformation of Drosophila virilis mediated by the transposable element hobo
E R Lozovskaya1, D I Nurminsky, D L Hartl
1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Abstract:
A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using a helper plasmid with an hsp70-driven hobo transposase-coding sequence. The rate of transformation was 0.5% per fertile GO animal. Three transgenic insertions were cloned and characterized and found to be authentic hobo insertions. These results, together with the known widespread distribution of hobo in diverse insect species, suggest that hobo and related transposable elements may be of considerable utility in the germline transformation of insects other than D. melanogaster.
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