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Related Experiment Videos

Apoptosis in glomerular sclerosis

H Sugiyama1, N Kashihara, H Makino

  • 1Third Department of Internal Medicine, Okayama University Medical School, Japan.

Kidney International
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

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Apoptosis, or programmed cell death, contributes to cell loss in glomerulosclerosis. Increased DNA fragmentation in kidney cells correlated with disease severity and worsening renal function in rat and human studies.

Area of Science:

  • Nephrology
  • Cell Biology
  • Pathology

Background:

  • Glomerulosclerosis involves progressive extracellular matrix buildup and glomerular cell loss.
  • The specific mechanisms of glomerular cell loss in glomerulosclerosis require further elucidation.

Purpose of the Study:

  • To investigate the role of glomerular cell apoptosis in the pathogenesis of glomerulosclerosis.
  • To assess the correlation between apoptotic cell markers and disease progression in kidney tissues.

Main Methods:

  • Utilized a rat remnant kidney model and human glomerular disease samples.
  • Employed electron microscopy to identify apoptotic cells in glomeruli, tubules, and interstitium.
  • Applied in situ nick end-labeling (NELA) to detect DNA fragmentation, a hallmark of apoptosis.

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Main Results:

  • Apoptotic cells were identified in multiple kidney compartments in the rat model.
  • Increased DNA fragmentation in glomeruli and tubules correlated with glomerulosclerosis progression.
  • NELA-positive cells in glomeruli significantly correlated with glomerulosclerosis severity and renal dysfunction in human diseases like IgA and lupus nephritis.

Conclusions:

  • Apoptosis plays a significant role in the cell deletion observed in various glomerular diseases.
  • The findings suggest apoptosis is a key contributor to the development and progression of glomerulosclerosis.