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Related Experiment Videos

Immortalization of epithelial cells

U Hopfer1, J W Jacobberger, D C Gruenert

  • 1Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106, USA.

The American Journal of Physiology
|January 1, 1996
PubMed
Summary

Powerful cell line isolation methods retain parent cell features, creating valuable models for tissue biology, disease study, and gene therapy screening. These cell lines are derived from primary cells or transgenic animals.

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Genetics

Background:

  • Advanced cell line isolation techniques now preserve crucial differentiated characteristics of parent cells.
  • Cell lines can originate from primary cells, early-passage cells, or transgenic animals harboring immortalizing genes.

Purpose of the Study:

  • To highlight the capabilities of modern cell line isolation methodologies.
  • To underscore the utility of established cell lines as research models.

Main Methods:

  • Development of cell lines from primary or early passage cells.
  • Utilization of transgenic animal models carrying immortalizing genes.
  • Selection of epithelial cell lines for specific cellular attributes like polarity and junction formation.

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Main Results:

  • Isolated cell lines successfully retain differentiated features of the parent cells.
  • Epithelial cell lines exhibit polar orientation, tight junction formation, and express differentiated markers.
  • These cell lines serve as effective models for diverse biological investigations.

Conclusions:

  • Modern cell line isolation techniques are highly effective in preserving cellular differentiation.
  • Established cell lines are versatile tools for studying tissue-specific cell biology, disease mechanisms, and therapeutic strategies like gene therapy.