Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gut proteolysis contributes essential amino acids during exercise

B D Williams1, R R Wolfe, D P Bracy

  • 1Metabolism Unit, Shriners Burns Institute, Galveston, Texas, USA.

The American Journal of Physiology
|January 1, 1996
PubMed
Summary

Exercise increases whole body leucine appearance, with gut leucine kinetics significantly elevated during moderate-intensity activity. This suggests gut protein contributes to muscle protein balance post-exercise.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short-term provision of moderate dietary starch alters fecal microbiota but does not exacerbate exercise-induced inflammation in yearling Quarter Horses.

Journal of equine veterinary science·2026
Same author

Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling.

American journal of physiology. Regulatory, integrative and comparative physiology·2017
Same author

Medication persistence in older women with osteoporosis: a pilot study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

AMP-activated protein kinase (AMPK)α2 plays a role in determining the cellular fate of glucose in insulin-resistant mouse skeletal muscle.

Diabetologia·2012
Same author

Regulation of glucose kinetics during exercise by the glucagon-like peptide-1 receptor.

The Journal of physiology·2012
Same author

Aldosterone decreases glucose-stimulated insulin secretion in vivo in mice and in murine islets.

Diabetologia·2011

Area of Science:

  • Exercise Physiology
  • Nutritional Biochemistry
  • Metabolic Research

Background:

  • Leucine kinetics are crucial for understanding protein metabolism.
  • Exercise significantly impacts amino acid utilization.
  • The role of gut-derived amino acids during exercise requires further elucidation.

Purpose of the Study:

  • To investigate the effects of moderate-intensity exercise on whole body, gut, liver, and splanchnic leucine kinetics.
  • To determine the fate of leucine during rest, exercise, and recovery phases.
  • To assess the contribution of gut protein to amino acid availability during physical exertion.

Main Methods:

  • Utilized arteriovenous difference and tracer dilution techniques.
  • Infused dogs with [1-13C]leucine to track kinetics.

Related Experiment Videos

  • Measured leucine kinetics during rest, 90 minutes of treadmill exercise, and 90 minutes of recovery.

    • Main Results:

    • Whole body leucine rate of appearance (Rai) increased significantly during late exercise compared to rest.
    • Gut leucine Rai also increased significantly during both early and late exercise.
    • Liver leucine Rai showed no significant change, while whole body leucine rate of disappearance (Rd) paralleled Rai.
    • Leucine Rd in the gut, liver, and splanchnic bed remained unchanged, indicating increased uptake in other tissues.

    Conclusions:

    • Exercise, particularly moderate-intensity, enhances whole body and gut leucine appearance.
    • Increased leucine uptake outside the gut, liver, and splanchnic regions suggests utilization by active skeletal muscle.
    • Gut protein-derived amino acids likely contribute to mitigating net muscle protein breakdown during and after exercise.