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Ion transport systems in human labial acinar cells
M Paulais1, I H Valdez, P C Fox
1Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
The American Journal of Physiology
|January 1, 1996
Summary
Human labial glands utilize sodium-potassium-chloride cotransporter activity for fluid secretion. This contrasts with other salivary glands, highlighting distinct ion transport mechanisms in human labial acini.
Area of Science:
- Physiology
- Cell Biology
- Ion Transport
Background:
- Salivary fluid secretion relies on intricate ion transport systems.
- Human labial glands present a unique model for studying salivary secretion mechanisms.
Purpose of the Study:
- To investigate the ion transport systems involved in human labial acini fluid secretion.
- To compare these mechanisms with those found in rat parotid acini.
Main Methods:
- Microfluorometric assays were employed to measure ion transport activities.
- Sensitivity to bumetanide and amiloride was used to identify Na(+)-K(+)-Cl(-) cotransport and Na+/H+ exchange, respectively.
- Effects of adrenergic and muscarinic stimulation were assessed.
Main Results:
- Human labial acini showed approximately 50% of the Na(+)-K(+)-Cl(-) cotransport and Na+/H+ exchange activity found in rat parotid acini.
- Minimal Cl-/HCO3- exchange activity was detected.
- Na+/H+ exchange was downregulated by beta-adrenergic and upregulated by muscarinic stimulation.
- Na(+)-K(+)-Cl(-) cotransport was marginally increased by beta-adrenergic stimulation and unaffected by muscarinic stimulation.
Conclusions:
- Basolateral Na(+)-K(+)-Cl(-) cotransport is the primary driver of chloride and fluid secretion in human labial acini.
- This suggests a distinct physiological mechanism compared to other salivary glands.