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Quantification of tumour-induced angiogenesis by image analysis
M Iwahana1, Y Nakayama, N G Tanaka
1Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Japan.
International Journal of Experimental Pathology
|June 1, 1996
Summary
This study developed quantitative image analysis techniques to measure tumor angiogenesis. Automated methods accurately assessed neovascularization in vivo and endothelial cell migration in vitro, aiding drug development.
Area of Science:
- Angiogenesis research
- Tumor biology
- Medical imaging analysis
Background:
- Tumor angiogenesis, the formation of new blood vessels, is crucial for tumor growth and metastasis.
- Accurate quantification of angiogenesis is essential for evaluating anti-cancer therapies.
Purpose of the Study:
- To develop and validate quantitative techniques for assessing in vivo and in vitro angiogenesis using an automated image analyzer.
- To evaluate the role of tumor cells in inducing neovascularization and endothelial cell migration.
Main Methods:
- In vivo: Quantification of neovascularization in mice bearing sarcoma 180 cells using image analysis after Laplacean transformation for contrast enhancement.
- In vitro: Automated counting of migrated endothelial cell nuclei induced by sarcoma 180 conditioned media using Giemsa staining and image analysis.
- Checkerboard analysis to determine the mechanism of endothelial cell migration (chemotaxis).
Main Results:
- Tumor cells significantly increased the neovascularized area in vivo (44.2 mm2) compared to controls (16.9 mm2).
- Automated image analysis efficiently quantified endothelial cell migration in vitro.
- Endothelial cell migration induced by tumor-conditioned media was confirmed to be chemotaxis.
Conclusions:
- The developed automated image analysis method provides a valuable tool for quantifying tumor angiogenesis.
- This quantitative approach aids in evaluating the efficacy of pharmacological agents on angiogenesis both in vivo and in vitro.