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Expression of TrkB receptor isoforms in the developing avian visual system

A S Garner1, H J Menegay, K L Boeshore

  • 1Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4975, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|March 1, 1996
PubMed
Summary

Novel brain-derived neurotrophic factor (BDNF) receptor variants in the avian visual system reveal diverse signaling roles. These TrkB receptor isoforms are expressed in retinal ganglion cells and the tectum, suggesting complex BDNF functions during development.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Brain-derived neurotrophic factor (BDNF) plays crucial roles in neuronal development and survival.
  • The TrkB receptor mediates BDNF signaling, but its diverse isoforms and functions in the developing visual system are not fully understood.

Purpose of the Study:

  • To characterize novel TrkB receptor transcripts in the developing avian visual system.
  • To investigate the expression patterns and potential roles of different TrkB receptor isoforms in the retina and tectum.

Main Methods:

  • In situ hybridization using an extracellular domain probe for TrkB transcripts.
  • cDNA cloning and reverse transcription-PCR (RT-PCR) analysis to identify receptor isoforms.
  • Sequencing of identified receptor isoforms to determine structural variations.

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Main Results:

  • TrkB receptor transcripts were localized to retinal ganglion cells and visual nuclei in the tectum.
  • Several TrkB receptor isoforms were identified, including those with deletions in the N terminus, ligand-binding domain, or juxtamembrane (JM) domain.
  • Novel JM insertion sequences, one with weak homology to beta-actin, were found in both kinase-containing (TK+) and kinase deletion (KD) isoforms.
  • TK+ receptor mRNA upregulation coincided with retinal ganglion cell (RGC) death, suggesting a role in RGC survival.
  • TrkB expression in the tectum indicates BDNF responsiveness in this structure.

Conclusions:

  • The developing avian visual system expresses diverse TrkB receptor isoforms with unique structural features.
  • BDNF signaling through TrkB may play roles in RGC survival and retinotectal trophic support.
  • Autocrine/paracrine signaling by BDNF within the retina and tectum is a possibility mediated by TrkB.