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Related Experiment Videos

Transcription activation in cells lacking TAFIIS

S S Walker1, J C Reese, L M Apone

  • 1Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA.

Nature
|September 12, 1996
PubMed
Summary

TFIID, a transcription factor, includes TBP and TAFIIs. In vivo studies show that many genes can be activated without functional TAFIIs, suggesting activators target other factors.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The general transcription factor TFIID is crucial for gene expression, comprising TBP and TAFIIs.
  • In vitro studies suggest TAFIIs are essential for activated transcription and direct targets of activator proteins.
  • The in vivo function of TAFIIs remains largely uncharacterized.

Purpose of the Study:

  • To investigate the in vivo role of TAFIIs in Saccharomyces cerevisiae transcription.
  • To determine if TAFIIs are obligatory for activated transcription in vivo.
  • To identify potential alternative targets of transcriptional activators.

Main Methods:

  • Systematic inactivation or depletion of six different yeast TAFIIs (yTAFIIs).
  • Analysis of transcriptional activation in yeast strains lacking functional yTAFIIs.

Related Experiment Videos

  • Comparison of yeast TFIID complex with higher eukaryotic TFIID.

    • Main Results:

    • Inactivation or depletion of six yTAFIIs, including the TBP-contacting core yTAFII, did not impair transcriptional activation.
    • Activated transcription of numerous genes occurred successfully even without functional yTAFIIS.
    • This indicates that yTAFIIS are not essential for all instances of in vivo activated transcription.

    Conclusions:

    • Activated transcription in vivo can proceed independently of functional yTAFIIS for many genes.
    • Transcriptional activator proteins may target other components of the transcription machinery in vivo.
    • These findings challenge the proposed obligatory role of TAFIIs as direct targets of activators in all contexts.