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Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface
1Pacific Northwest National Laboratory, Richland, Washington 99352, USA. mf_romine@pnl.gov
Applied and Environmental Microbiology
|July 1, 1996
Abstract:
Four plasmids, each encoding a combination of either an Escherichia coli or Pseudomonas putida promoter and either toluene dioxygenase or toluene monooxygenase, were electroporated into five bacterial strains isolated from sediments found at depths of 91 to 295 m. Four of these engineered bacterial strains demonstrated both toluene and trichloroethylene degradation activities.